Keywords
Vriesea hieroglyphica
Brazilian bromeliads
Atlantic rain forest canopy
How to Cite
Abstract
Seeds were used for establishment of a sterile culture of Vriesea fosteriana and Vriesea hieroglyphica, two endangered ornamental Brazilian bromeliads. Large number of plantlets could be obtained from embryos of Vriesea hieroglyphica when cultivated in Knudson basal medium. In contrast, Vriesea fosteriana the addition of hormones was required to produce protuberances from which buds were formed. In this process, new leafy shoots can be formed continuously, providing expiants to continue the multiplication. Entire leaves removed from aseptically-grown seedlings of Vriesea fosteriana were also used as a source of expiants. After twenty days of culture, protuberances were formed at the basal part of them and shoot-buds appeared after one month of culture. For rooting, two different auxins were necessary depending on the species. The rooted shoots were planted in pots and grown in the greenhouse. All plants survived and looked phenotypically normal. This methodology provided a large number of plants, preserving the genetic diversity of the species. This feature is essential for any replacement program that makes use of micropropagated plants.
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