MORPHOLOGICAL, ENZYMATIC AND MOLECULAR CHARACTERIZATION OF MELOIDOGYNE ARENARIA FROM ANUBIAS BARTERI VAR. CALADIITOLIA IN CHINA [CARACTERIZACIÓN MORFOLÓGICA, ENZIMÁTICA Y MOLECULAR DE MELOIDOGYNE ARENARIA PROCEDENTE DE ANUBIAS BARTERI VAR. CALADIITOL

Abstract

Studies were conducted to characterize morphological, enzymatic and molecular profiles of Meloidogynearenaria from Anubias barteri var. caladiitolia in China. This nematode population can cause visible galls onthe roots of the infected plants and the female vulval region was typically surrounded by an egg mass. Mostmorphometrics of the population fit within the ranges reported for M. arenaria. The isozyme phenotypes foresterase and malate dehydrogenase and a single 420 bp-band produced by sequencing characterized amplifiedregions marker with species-specific primers Far/Rar matched those reported for M. arenaria. The sequences ofpartial 18S small subunit rRNA gene and 28S D2/D3 expansion segment all share identities of 99% with other M.arenaria population (AY942623), M. javanica (AY942626) and M. incognita (JQ806343).