The Instituto Nacional de Biodiversidad (INBio), in collaboration with the Nematology Laboratory at Universidad Nacional (U.N.A) of Costa Rica, conducted a nematode inventory of Costa Rican wild lands. Hundreds of samples were randomly collected for nematode analyses and thousands of specimens were permanently mounted on Cobb slides. A total of 74 families, 231 genera and 105 species has been detected so far. Dorylaimida was the dominant order both in number of specimens and genera, while the least dominant orders were Aphelenchida, Monhysterida and Desmocolecida. Some nematodes were widely distributed across all microhabitats, others appeared be restricted to a specific microhabitat or ecosystem. Basic and advanced reports regarding nematode inventory in Costa Rican protected areas can be requested through the web page of INBio (http://atta.inbio.ac.cr).