DISTRIBUTION OF STAGES AND <I>IN VITRO</I> LARVAL HATCHING IN <I>GLOBODERA ROSTOCHIENSIS</I> CYSTS
Keywords:density, hatching, population structure, viability
AbstractDesgarennes, D., G. Carrion, A. E. Nunez-Sanchez, and M. C. Nunez-Camargo. 2006. Distribution of stages and in vitro larval hatching in Globodera rostochiensis cysts. Nematropica 36:251-260. The classification of cysts as young (YC), mature (MC), and old (OC) on the basis of cyst wall color was shown to be a practical tool for the diagnosis of population status. Also important are differences in the stages that occur within cysts (eggs, J1, and J2), as they too are linked to differences in color. These data, from an ecological point of view, help us understand population structure. The density recorded for Los Pescados, Perote in the state of Veracruz, Mexico in one kilogram of soil was 2974 YC, 3004 MC, and 221 OC. On average, young cysts have 55-63% viable eggs, 37% of which contain a vitellus, without a developing embryo; MC have 38-54% viability, 39% of which are J1, with J2 also observed; in OC, 98% are empty eggs and have 2% viability with J1 and J2 juveniles. The greatest degree of hatching was observed in MC (19%), with juveniles hatching only four hours after being exposed to root exudates.