The suitability of 38 host lines belonging to 12 crop plant species as hosts of Ditylenchus destructor was tested under greenhouse conditions. All maize (Zea mays) and grain sorghum (Sorghum bicolor) host lines and one of the four tobacco (Nicotiana tabacum) host lines supported as many D. destructor per 5 g of root as 'Sellie' peanut (Arachis hypogaea). All host lines of wheat (Triticum aestivum), sunflower (Helianthus annuus), lupin (Lupinus albus), drybean (Phaseolus vulgarus), cowpea (Vigna unguiculata), soybean (Glycine max), alfalfa (Medicago sativa), cotton (Gossypium hirsutum), pea (Pisum sativum) and three of the four tobacco host lines supported less D. destructor per 5 g of root than 'Sellie' peanut. In all host lines, including 'Sellie' peanut, the number of D. destructor recovered per root system was lower than the number of nematodes used for inoculation.