Caryo-phenotypes of Ditylenchus dipsaci in Chile
AbstractFour caryotypes (n=6, 2n=24, 4n=54 and 4n = 60) of the stem nematode, Ditylenchus dipsaci (Kuehn) Filipjev, are known in Europe (D'Addabbo Gallo et al., 1982). Chromosome numbers were counted in two populations of the stem nematode, collected in Chile, to ascertain their similarity with the European ones. One of the populations was collected from an alfalfa field, near Santiago in the Region Metropolitana by lng. Agr. A. Guinez, and the other from an onion field, near Valparaiso in the V Region by lng. Agr. E.M. Tapia V. Nematodes were extracted from plant tissues; male gonads were dissected and stained in acetic orcein, propionic orcein and Feulgen for chromosome counts; measurements were taken of specimens of either sex fixed in hot 5% formalin and mounted in anhydrous glycerine. Chromosome counts revealed the presence of two caryotypes: one associated with alfalfa, in which 2n= 12, and the other associated with onion, in which 2n = 24. Biometric characters of the two populations are listed in Table 1. Damage caused by D. dipsaci on crops in South America has only recently been reported. These observations would indicate that Chilean populations of this species might have a North American or European origin (Hooper, 1972).