Randomly Amplified Polymorphic DNA Differs with Burrowing Nematode Collection Site, but not with Host Range

Authors

  • D. H. Marin
  • D. T. Kaplan
  • C. H. Opperman

Keywords:

banana, biogeography, citrus, genetic diversity, molecular biology, musa, nematode, radopholus, rapd

Abstract

The genetic variability of 12 burrowing nematode (Radopholus sp.) isolates from Central America, the Caribbean, and Florida, and one isolate from Ivory Coast were compared with RAPD analysis. A high degree of genetic similarity ( 0.82) was determined for isolates from the Western Hemisphere. Genome similarity was greatest among isolates collected within a country. Among isolates collected in Central America and the Caribbean, burrowing nematodes from Belize and Guatemala were genetically more distant. However, the genome of the isolate from Ivory Coast was most dissimilar ( 0.30). These results suggest that African and American burrowing-nematode isolates may have had different origins or that they have been geographically isolated for a sufficient amount of time to have accumulated genetic changes detectable by RAPD analysis. No relationship was found between the genomic similarity and extent of reproduction or damage to banana or citrus roots. Morphometric analysis involving eight of the isolates indicated that they were morphologically identical and values for morphometric parameters were well within the range previously published for banana and citrusparasitic burrowing nematodes.

Downloads

Published

1999-06-15

Issue

Vol. 31, No. 2 (June 1999)

Section

Articles

License

Copyright and Permissions

All material published by the Society of Nematologists (SON), except for papers prepared by United States and Canadian government employees, is copyrighted and protected under the U.S. copyright law. Under the Copyright Act of 1976, the term of copyright for materials registered by an organization is 75 years from the date first published. Before publishing any manuscript, SON requires that authors transfer full and complete ownership of any copyright to SON by signing a JON Page Charge/Copyright Form (.pdf). SON then registers the copyright. Subsequent use of published materials requires written permission from the SON and may be obtained by contacting the current Editor-in-Chief and state where and how the material will be used.

The author warrants that the article is an original work not published elsewhere in whole or in part, except in abstract form, and that the author has full power to make this grant. If portions of the article have been published previously, then the author warrants that permission has been obtained from the copyright holder and the author will submit a copy of the permission release with this copyright transfer form.

SON shall claim no proprietary right other than copyright. Authors and coauthors retain the right to revise, adapt, modify, or otherwise use all or part of the article in future works of the author(s), such as press releases, lectures, and reviews, provided that all such use is for the personal noncommercial benefit of the author(s). All patent rights are retained by the author(s).