The Immunofluorescent Localization of Subventral Pharyngeal Gland Epitopes of Preparasitic Juveniles of Heterodera glycines Using Laser Scanning Confocal Microscopy

Authors

  • William G. T. Willats
  • Howard J. Atkinson
  • Roland N. Perry

Abstract

Laser scanning confocal microscopy (LSCM) was used to localize the reactivity of a monoclonal antibody (Sv2) that binds to the subventral pharyngeal glands of preparasitic juveniles of Heterodera glycines. The greater resolution, magnification, and image analysis of LSCM compared with conventional epifluorescent microscopy enabled Sv2 binding to be localized much more precisely to the periphery of the secretory granules. A linear increase of about 55% in fluorescent intensity was found over a 23-[mu]m length of subventral pharyngeal gland just distal to the terminal ampullae. LSCM is a rapid and effective technique for precise immunolocalization of epitopes. Key words: confocal microscopy, Heterodera glycines, nematode, monoclonal antibody, secretory granule, subventral pharyngeal gland.

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Published

1995-06-15

Issue

Vol. 27, No. 2 (June 1995)

Section

Articles

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