Ultrastructure of Coelomocytes in Sphaerolaimus gracilis de Man, 1876 (Nematoda)


  • Tuomo A. Turpeenniemi


The structure of coelomocytes in the adenophorean aquatic nematode Sphaerolaimus gracilis de Man 1876 was studied with light and electron microscopes. Acid phosphatase and catalase activities were demonstrated by electron microscopy. Two pairs of coelomocytes occurred laterally posterior to the esophagointestinaljunction. The anterior pair of the coelomocytes, with the renette cell and gonad, lay in either the left or the right lateral side of the body. The posterior pair of coelomocytes was in the opposite side of the body, usually posterior to the renette. A long, thin, cell-extension-like structure appeared to originate from the coelomocytes. Coelomocytes were characterized by specialized organelles (CC-organelle) and large vacuoles. The CC-organelle contained crystalline structures like those in peroxisomes. Acid phosphatase and catalase activities were detected in the matrix of CC-organelles and catalase activity in the vacuoles. It was assumed that vacuoles originate from the CC-organelles. Coelomocytes showed pinocytotic activities, and numerous vesicles were observed between the cell membranes and the vacuoles. Key words: acid phosphatase, catalase, CC-organelle, coelomocyte, enzyme, nematode, Sphaerolaimus gracilis, ultrastructure.