Effects of Inoculum Density and Egg Age on Establishment of Globodera rostochiensis Populations


  • B. B. Brodie
  • M. L. Brucato


The establishment of Globodera rostochiensis Rol populations was examined under greenhouse conditions. The probability of G. rostochiemis population establishment was calculated from the number of plants that produced new cysts with viable eggs following inoculation with various numbers of eggs of different ages. Probability of population establishment was positively correlated with inoculum density but was not affected by the age of eggs used in these experiments. The probability of G. rostochiensis establishment ranged from 5% at densities of 2 eggs/pot to 100% at densities of 25 eggs/pot or greater. At densities of 3 eggs/pot and beyond, there was no correlation between inoculum density and the number of viable eggs/new cyst. Also, the number of plants that produced new cysts was a function of inoculum density and not age of eggs. Juveniles from eggs 1 year old or older were equally as infective as were those from eggs in newly developed cysts (4 months old). Key words: Globodera rostochiensis, golden nematode, nematode spread, population establishment, potato, Solanum tuberosum.