Sterile Culture of Rotylenchulus reinformis on Tomato Root with Gellan Gum as a Supporting Medium

Authors

  • Melissa J. Eyre and Edward P. Caswell

Abstract

Rotylenchulus reniformis was repeatedly propagated in sterile excised tomato roots growing on modified White's medium with gellan gum as the support. Gellan gum provided an optically clear support medium that could be liquified by adding 5 mM disodium ethylenediaminetetraacetate (EDTA) to facilitate nematode extraction. Liquefaction of the gellan-gum medium by EDTA allowed efficient recovery of eggs and vermiform stages of R. reniformis. Extraction efficiency was quantified with Radopholus similis as a test organism. The efficiency of extracting R. similis from the gellan gum did not vary with the concentrations of EDTA tested. Key words: culture, disodium ethylenediaminetetraacetate (EDTA), gellan gum, Lycopersicon esculentum, Rotylenchulus reniformis, sterile culture, technique, tissue culture, tomato.

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Published

1991-04-15

Issue

Vol. 23, No. 2 (April 1991)

Section

Articles

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