Composition of High Molecular Weight Excretions-Secretions from Infective Larvae of Meloidogyne javanica
Abstract
Infective larvae (J2) of Meloidogyne javanica were incubated in distilled water for up to 14 days, and their high molecular weight ( 1,000 daltons) excretions-secretions (ES) were isolated and partially characterized. The ES consisted of a mixture of proteins, glycoproteins, and proteoglycans or polysaccharides as revealed by differential staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compositional analysis. Carbohydrate, with approximately equal amounts of neutral monosaccharides and hexosamines, was the major constituent of the ES, with only low levels of protein detected. Acidic sugar residues, including sialic acids, were not detected. Key words: carbohydrate composition, excretion-secretion, infective larval stage, Meloidogyne javanica, root-knot nematode.Downloads
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