Sterol Composition of the Corn Cyst Nematode, Heterodera zeae, and Corn Roots

Authors

  • David J. Chitwood
  • Paula A. Hutzell
  • William R. Lusby

Abstract

Sterols from free sterol and steryl ester fractions from Heterodera zeae and from total lipids of Zea mays roots were analyzed by gas-liquid chromatography (GLC) and by GLC-mass spectrometry. The major free sterols of H. zeae were 24-ethylcholesterol (54.4% of total free sterol), 24-ethylcholesta-5,22-dien-3[beta]-ol (13.3%), 24-methylcholesterol (12.5%), and cholesterol (7.2%). The same four sterols comprised 34.6%, 7.2%, 30.3%, and 18.6%, respectively, of the esterified sterols of H. zeae. Corn root sterols included 46.6% 24-ethylcholesta-5,22-dien-3[beta]-ol, 16.7% methylcholesterol, 16.4% cycloartenol, 12.7% 24-ethylcholesterol, and 0.5% cholesterol. The sterol 24-composition of H. zeae differed greatly from that of the only other cyst nematode previously investigated, Globodera solanacearum. Key words: maize, mass spectrometry, steryl esters, Zea mays.

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Published

1985-01-15

Issue

Vol. 17, No. 1 (January 1985)

Section

Articles

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