Transport of Palmitic Acid Across the Tegument of the Entomophilic Nematode Romanomermis culicivorax
AbstractRomanomermis culicivorax juveniles, dissected out of Aedes aegypti larvae 7 days after infection, were incubated under controlled conditions in isotonic saline containing¹[sup4]C-U-palmitic acid to investigate the nature of the transport mechanism(s) used by the nematode for transcuticular uptake of palmitic acid. Net uptake of the isotope by the nematode was of a logarithmic nature with respect to time. Uptake of palmitic acid was accomplished by a combination of diffusion and a mediated process which was substrate saturable and competitively inhibited by myristic and stearic acids. Both 2,4-dinitrophenol and ouabain inhibited uptake of palmitic acid and thus supported the hypothesis that the carrier system is of the active transport variety and is coupled to a Na[sup+]K[sup+] ATPase pump. Key words: active transport, Aedes aegypti, diffusion, fatty acid, lipid, mediated transport, mermithid, nutrition, transtegumentary uptake.
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