Histopathology of Selected cultivars of Tobacco infected with Meloidogyne species


  • C. Sosa-Moss
  • K. R. Barker
  • M. E. Daykin


Rates of nematode penetration and the histopathology of root infections in fluecured tobacco cultivars 'McNair-944,' 'Speight G-28,' and 'NC-89' with either Meloidogyne arenaria, M. incognita, M. hapla, or M. javanica were investigated. Penetration of root tips by juveniles of all species into the M. incognita-resistant NC-89 and G-28 was much less than that on the susceptible McNair-944. Few juveniles of M. incognita were detected in resistant cultivars 7 and 14 days after inoculation. Infection sites exhibited some cavities and extensive necrotic tissue at 14 days; less necrotic tissue and no intact nematodes were observed 35 days after inoculation. Although some females of M. arenaria reached maturity and produced eggs, considerable necrosis was induced in the resistant cultivars. Meloidogyne hapla and M. javaniea developed on all cultivars, but there was necrotic tissue at some infection sites in the resistant cultivars. The occurrence of single multistructured nuclei in the syncytia of most M. hapla infections differed from the numerous small nuclei found in syncytia caused by the other three species. Key words: Nicotiana tabacum, root knot, resistance, M. arenaria, M. incognita, M. hapla, M. javanica.