Cultivation of the Pine Wilt Nematode, Bursaphelenchus xylophilus, in Axenic Culture Media

Authors

  • R. I. Bolla
  • W. Jordan

Abstract

The pine wilt nematode, Bursaphelenchus xylophilus, has been cultured axenically in vitro on soy peptone/yeast extract or modified Caenorhabditis medium supplemented with cholesterol and hemoglobin. Although growth, development and reproduction were best in soy peptone/yeast extract medium, satisfactory population size increases were observed in the chemically defined Caenorhaditis medium. Key words: axenic culture, Bursaphelenchus xylophilus, in vitro culture, pine wilt disease.

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Published

1982-07-15

Issue

Vol. 14, No. 3 (July 1982)

Section

Articles

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